Application note
Modified Oligonucleotides: Planning the ChemistryMap
Research considerations for specifying backbone, sugar, base, linker, and terminal modifications.
Why the map matters
A modification name is not a complete specification. Position, neighbouring bases, strand orientation, purification, and downstream conditions can change feasibility and handling.
Information to record
- Full sequence with every modified residue or linkage annotated
- Terminal groups, linkers, labels, conjugates, or spacers
- Intended application and any buffer or solvent constraint
- Requested quantity, purity, and final format
Design boundary
Backbone or sugar changes may alter nuclease resistance, binding, uptake, solubility, or assay behaviour, but the direction and magnitude are sequence- and system-dependent. Experimental performance is not guaranteed by synthesis.
Feasibility review
Complex chemistry combinations may require a revised design. The accepted modification map and analytical documentation are listed in the quotation.
Turn this into a project
Bring your sequences, samples, or brief and we will confirm scope before quoting.