Guide
Oligo DesignGuide
Practical checks to complete before submitting primers, probes, or other DNA and RNA oligonucleotide sequences.
Start with the assay
Record the intended reaction, target sequence, strand orientation, and instrument or detection chemistry before selecting length or modifications.
Sequence review
- Check that names and sequences are unique and unambiguous
- Review GC balance, repetitive motifs, homopolymers, and predicted secondary structure
- For primer pairs, examine compatibility and unintended products in the relevant reference
- For probes, record the exact reporter, quencher, linker, and attachment positions
Purification and format
Purification needs depend on sequence length, chemistry, and downstream sensitivity. State the application rather than selecting a grade from a generic rule.
What to submit
Provide a sequence table, target or accession information, requested chemistry, scale or quantity, and any required delivery format. Experimental performance remains dependent on assay design and customer validation.
Ready when you are
Turn this into a project
Bring your sequences, samples, or brief and we will confirm scope before quoting.